Detection of Helicobacter pylori in water by direct PCR |
| |
Authors: | Benson J A Fode-Vaughan K A Collins M L P |
| |
Institution: | Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53201, USA. |
| |
Abstract: | AIMS: This paper demonstrates a rapid, simple method for the detection of Helicobacter pylori in water that eliminates the need for recovery of cells or DNA extraction prior to PCR. METHODS AND RESULTS: Direct polymerase chain reaction (DPCR) with primers specific for H. pylori ureA (urease, subunit A) were used to detect H. pylori added to groundwater. DPCR also detected H. pylori in a naturally contaminated water sample. CONCLUSIONS: DPCR should provide an improved method to assess contamination of water by H. pylori. SIGNIFICANCE AND IMPACT OF THE STUDY: This simple, rapid method for detection of H. pylori in water will provide an improved means to investigate the possible role of water as a disease vector. |
| |
Keywords: | detection direct PCR DPCR Helicobacter pylori PCR |
本文献已被 PubMed 等数据库收录! |
|