In vitro culture selection increases glyphosate tolerance in barley |
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Authors: | Maria Concepción Escorial Hortensia Sixto José-Maria García-Baudin Mariá-Cristina Chueca |
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Institution: | (1) Area Protección vegetal CIT-INIA, Carretera La Coruña, 28040 Madrid, Spain |
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Abstract: | In vitro culture of barley calluses has been used to produce plants with increased glyphosate tolerance. Calluses from immature embryos of barleyHordeum vulgare L. ( Jeff ) were cultured on Murashige and Skoog medium with 10-6, 10-5, 10-4, 5×10-4, 10-3, or 10-2M glyphosate for one, four or thirty months. Plants were regenerated from calluses maintained in glyphosate medium at 10-6, 10-5 or 10-4M for four months, at 10-5 or 5×10-4M for one month and at 10-5M for thirty months. The progeny of each regenerated plant was analyzed for response to glyphosate. Some progenies showed increased tolerance to glyphosate. |
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Keywords: | cereal herbicide-tolerance Hordeum vulgare somaclonal variation |
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