Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive <Emphasis Type="Italic">Bacillus subtilis</Emphasis> 1012 for the potential application as vaccines for immunotherapy of atopic allergy |
| |
Authors: | Nicola?Ilk Christian-Thomas?Schumi Barbara?Bohle Email author" target="_blank">Eva?Maria?EgelseerEmail author Uwe?B?Sleytr |
| |
Institution: | (1) Department of NanoBiotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, A-1190, Vienna, Austria;(2) Christian Doppler Laboratory for Immunomodulation, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology & Immunology, Medical University of Vienna, A-1090 Vienna, Austria |
| |
Abstract: | Background Genetic fusion of the major birch pollen allergen (Bet v1) to bacterial surface-(S)-layer proteins resulted in recombinant
proteins exhibiting reduced allergenicity as well as immunomodulatory capacity. Thus, S-layer/allergen fusion proteins were
considered as suitable carriers for new immunotherapeutical vaccines for treatment of Type I hypersensitivity. Up to now,
endotoxin contamination of the fusion protein which occurred after isolation from the gram-negative expression host E. coli had to be removed by an expensive and time consuming procedure. In the present study, in order to achieve expression of pyrogen-free,
recombinant S-layer/allergen fusion protein and to study the secretion of a protein capable to self-assemble, the S-layer/allergen
fusion protein rSbpA/Bet v1 was produced in the gram-positive organism Bacillus subtilis 1012. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|