Genetic heterogeneity of heat shock protein synthesis as a factor determining the resistance to stressors in mammalia |
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Authors: | A L Pukhalsky G V Shmarina I V Kapustin S V Stukalov D A Pukhalskaya V A Alioshkin |
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Institution: | 1.Research Centre for Medical Genetics,Moscow,Russia;2.G.N. Gabrichevsky Institute of Epidemiology and Microbiology,Moscow,Russia |
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Abstract: | The relationship between the levels of 70 kDa family heat shock protein (Hsp) synthesis and lymphocyte sensitivity to stressors
was investigated. Lymphocyte cultivation in mitogen deprived culture medium and/or the cell treatment with alkylating agents
have been used as a stress challenge. Model experiments with two inbred murine strains genetically contrasting by the sensitivity
to alkylating agents demonstrated that the basic level of Hsp synthesis depends on genotype. The quantity Hsp70 mRNA, as well
as intracellular level of the proteins, in BALB/c was significantly higher than those in C57BL/6 mice. The mice, which were
characterized by higher Hsp levels, demonstrated higher resistance to alkylating agent action. The induction of surplus amount
of Hsp by heat shock increased the cell resistance to an alkylating agent melphalan. Lymphocyte isolated from high Hsp producers
BALB/c mice were more resistant to apoptotic signals induced by mitogen deprivation. |
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