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Hemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides |
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| Authors: | Dahai Gao Nirmal Uppugundla Shishir PS Chundawat Xiurong Yu Spencer Hermanson Krishne Gowda Phillip Brumm David Mead Venkatesh Balan Bruce E Dale |
| Affiliation: | 1.Biomass Conversion Research Lab (BCRL), Department of Chemical Engineering and Materials Science,Michigan State University,Lansing,USA;2.Lucigen Corporation,Middleton,USA;3.Great Lakes Bioenergy Research Center (GLBRC), 164 Food Safety and Toxicology Building,Michigan State University,East Lansing,USA;4.JiLin Rorgoo Renewable Energy Development Co Ltd,PR China |
| Abstract: | BackgroundHigh enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the enzyme cocktail for specific types of pretreated biomass allows for a significant reduction in enzyme loading without sacrificing hydrolysis yield. This is especially important for alkaline pretreatments such as Ammonia fiber expansion (AFEX) pretreated corn stover. Hence, a diverse set of hemicellulases supplemented along with cellulases is necessary for high recovery of monosaccharides. |
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