猕猴精子优选及体外获能的研究

选择活率高的精子并进行体外获能是开展猕猴体外受精研究的必要程序, 是研究猕猴受精生物学的重要手段。本实验采用上浮法和Percoll 梯度离心法对猕猴精液进行了优选, 并对处理后的精子形态正常率、精子活率、密度及受精率作了比较, 发现二者差异不显著; 用dbcAMP 和咖啡因使精子获能, 发现只有两种获能剂同时存在才能使猕猴精子获能并使卵母细胞受精。结论为: 上浮法和Percoll 法都是有效的精子优选法, 对受精率的影响差异不显著; dbcAMP 和咖啡因在猕猴精子体外获能时缺一不可。

Motile Sperm Separation and Sperm Capacitation in Rhesus Monkeys(Macaca mulatta)

Selection of fine quality rhesus monkeys'semen is needed for in vitro fertilization protocol, which is an important method for studying the biology of monkey reproduction. Semen was separated by the swim up and Percoll density gradient centrifugation methods, after which intact sperm rate, motility rate, concentration of sperm and fertility rate showed no great differences. Caffeine and dbcAMP were used during sperm capacitation, the result showed that rhesus oocyte can be fertilized when both caffeine and dbcAMP are added. The conclusion is that both caffeine and dbcAMP are necessary for inducing sperm capacitation, which affects the ability of sperm to bind to the zona and to undergo the acrosome reaction.