Unstable visible mutations induced in Drosophila melanogaster by injections of oncogenic virus DNA into the polar plasm of early embryos |
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Authors: | Karlen G. Gazaryan Sergei D. Nabirochkin Elena N. Shibanova Alexandr G. Tatosyan Feodor L. Kisselev Nune S. Ambartsumian Tom I. Tikhonenko Victor A. Goltzoy |
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Affiliation: | (1) Institute of Molecular Genetics of USSR Academy of Sciences, 123182 Moscow, USSR;(2) Embryology Department of Moscow State University, 117234 Moscow, USSR;(3) Cancer Research Center, USSR Academy of Medical Sciences, 115478 Moscow, USSR;(4) Institute of Experimental Biology of Armenian Academy of Sciences, 375019 Erevan, USSR;(5) Institute of Applied Molecular Biology of Academy of USSR Agricultural Sciences, Moscow, USSR |
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Abstract: | Summary When RSV DNA cloned in pBR 322 or DNA of simian adenovirus Sa7 (C8) is injected into the pole plasm of embryos of various Drosophila stocks, the progeny of 1–70% of the surviving flies display visible mutations. The mutagenesis is partially directed: the loci mutating due to retrovirus and adenovirus DNA do not everlap. The majority of resulting mutants are characterised by high instability: reversions and new mutations occur in them, which sometimes spread over the whole population(explosive instability). The injected sequences are revealed by dot-hybridization in the DNA of many mutant strains, but only rarely by Southern blotting procedures. The results show that the microinjection of oncovirus DNA into embryos is an approach for obtaining highly unstable strains even from wildtype stable Drosophila stocks without crosses with MR lines or the introduction of P elements. The sets of unstable mutations induced by oncovirus DNA is different from those in hybrid dysgenesis. |
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Keywords: | Oncovirus DNA Embryo Microinjection Unstable mutations |
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