不同剂量链脲佐菌素腹腔注射制备大鼠糖尿病心肌病模型的病理学观察

建立糖尿病性心肌病（DCM）大鼠模型,观察不同剂量链脲佐菌素（STZ）单次腹腔注射后大鼠心肌和胰腺的病理学变化。用STZ 50 mg/kg、55 mg/kg、60 mg/kg 3种剂量单次腹腔注射,制备糖尿病大鼠模型;以柠檬酸三钠-柠檬酸缓冲液腹腔注射,作为对照。72 h后,测空腹血糖及做口服葡萄糖耐量实验（OGTT）;3周后,HE染色观察各组大鼠胰腺和心肌形态学变化,Masson三色染色观察心肌纤维化改变。OGTT和空腹血糖显示3组存活大鼠糖尿病均成模;3周末,50 mg/kg和55 mg/kg剂量死亡率为25%;60 mg/kg剂量高,达到75%;HE染色显示55 mg/kg剂量组大鼠胰岛明显萎缩,轮廓不清晰,胰岛细胞数量少,心肌细胞肥大、排列紊乱,细胞间隙增大,并有炎症细胞浸润;50 mg/kg组胰岛和心肌也有变化,但无55 mg/kg组明显。心肌Masson染色显示55 mg/kg组心肌内胶原组织明显增多,排列紊乱,分布不均。55 mg/kg剂量的STZ单次注射大鼠腹腔,造模3周可以建立较明显的DCM模型,可为DCM的组织病理学和实验研究提供一个较好的动物模型。

Pathological observation of diabetic cardiomyopathy with rats by intraperitoneal injection different doses of STZ

To observe pathological changes of myocardium and pancreas in rats through establishing a rat model of diabetic cardiomyopathy with a single intraperitoneal injection of different doses of streptozotocin（ STZ）,SD rats were injected intraperitoneally with 50 mg/kg,55 mg/kg and 60 mg/kg STZ once to evoke the DM rats model. The control group only received an injection of 55 mg/kg trisodium citrate buffer. 72 hours after injection,fasting plasma glucose and oral glucose tolerance test（ OGTT） were performed. The pancreas and myocardium of rats were stained with hematoxylin and eosin（ HE） after 3 weeks. Myocardial fibrosis was described by Trichrome-masson staining. Result showed that OGTT and fasting plasma glucose showed all survival rats progress to full type 1 diabetes. At the end of the third week,mortality 50 mg/kg and 55 mg/kg STZ was 25%,and one of 60 mg/kg STZ reached 75%. HE staining revealed that the reduction of islets,fuzzy boundary,loss of B cells,and myocardial cells hypertrophic,disarranged,intercellular space,infiltration of inflammatory cells in 55 mg/kg STZ group,while the change of islets and myocardium was not obvious in 50 mg/kg STZ group.