| 气相色谱法测定发酵液中胆固醇的含量 |
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| 引用本文： | 刘长建,姜波,安晓雯,赵轶男,刘秋.气相色谱法测定发酵液中胆固醇的含量[J].微生物学杂志,2009,29(6):97-99. |
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| 作者姓名： | 刘长建 姜波 安晓雯 赵轶男 刘秋 |
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| 作者单位： | 大连民族学院,生命科学学院,辽宁,大连,116600 |
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| 基金项目： | 国家自然科学基金，NSFC-KOSEF国际合作项目 |
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| 摘 要： | 气相色谱法测定了发酵液中胆固醇的含量。采用Rtx-1（100%二甲基聚硅氧烷）毛细管色谱柱、FID检测器进行分析测定。当胆固醇浓度在0～200μg/mL范围内与色谱峰面积呈良好线性关系（r=0.9981）。方法的平均回收率为99.38%,相对标准偏差为0.75%（n=8）,最低检出限可达到0.816μg/mL。气相色谱法作为发酵液中胆固醇含量的快速测定方法,具有试剂用量少、操作简单、结果准确等特点。
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| 关 键 词： | 气相色谱法 胆固醇 发酵液 |
Content Determination of Cholesterol in Fermentation Solution by Gas Chromatography |
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| LIU Chang-jian,JIANG Bo,AN Xiao-wen,ZHAO Yi-nan,LIU Qiu.Content Determination of Cholesterol in Fermentation Solution by Gas Chromatography[J].Journal of Microbiology,2009,29(6):97-99. |
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| Authors: | LIU Chang-jian JIANG Bo AN Xiao-wen ZHAO Yi-nan LIU Qiu |
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| Institution: | (Coll.of Life Sci.,Dalian Nationalities Univ.,Dalian 116600) |
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| Abstract: | Content of cholesterol in fermentation solution was determined by gas chromatography. FID and Rtx-1 (100% dimethyl polysiloxane) capillary column were used to carry out the analysis and tests. When cholesterol concentration was in fine linear relation (r=0.998 1) with chromatogram peak area within the range of 0～200 μg/mL. The average recovery rate of cholesterol was 99.38% with the relative standard deviation as low as 0.75% (n=8). Determination limit of this method could amount to 0.816 μg/mL. Gas chromatography as a rapid testing method for cholesterol content in fermentation solution possessed traits of small amount of reagent, easy operation and accurate results etc. |
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| Keywords: | gas chromatography cholesterol fermentation solution |
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