Cloning and sequencing of the gene encoding the cell surface glycoprotein of Haloarcula japonica strain TR-1 |
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Authors: | Hiromichi Wakai Satoshi Nakamura Hiroshi Kawasaki Kei Takada Shin Mizutani Rikizo Aono Koki Horikoshi |
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Institution: | (1) Department of Bioengineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama-shi, Kanagawa 226, Japan, Tel. +81-45-924-5765; Fax +81-45-924-5819; e-mail: snakamur@bio-titech.ac.jp, JP |
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Abstract: | The triangular disk-shaped halophilic archaeon Haloarcula japonica strain TR-1 has a glycoprotein on its cell surface. The complete gene encoding the cell surface glycoprotein (CSG) was cloned
and sequenced. The gene has an open reading frame of 2586 bp, and a potential archaeal promoter sequence approximately 150
bp upstream of the ATG initiation codon. The mature CSG is composed of 828 amino acids and is preceded by a signal sequence
of 34 amino acid residues. A hydropathy analysis showed a hydrophobic stretch at the C-terminus, that probably serves as a
transmembrane domain. The amino acid sequence of the Ha. japonica CSG showed 52.1% and 43.2% identities to those from the Halobacterium halobium and Haloferax volcanii CSGs, respectively. Five potential N-glycosylation sites were found in the mature Ha. japonica CSG, sites that were distinctly different from those in Hb. halobium and Hf. volcanii. The Ha. japonica CSG gene was expressed in Escherichia coli.
Received: 20 September 1996 / Accepted: 9 October 1996 |
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Keywords: | Halophilic archaea Surface layer Cell surface glycoprotein Haloarcula japonica Polymerase chain reaction Gene cloning |
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