Long-range physical mapping of the alpha-amylase-1 (alpha-Amy-1) loci on homoeologous group 6 chromosomes of wheat |
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Authors: | W. Y. Cheung S. Chao M. D. Gale |
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Affiliation: | (1) Cambridge Laboratory, Colney Lane, NR4 7UJ Norwich, UK;(2) Present address: Department of Agronomy, University of Missouri, Curtis Hall, Columbia, 65211 Missouri, MO, USA |
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Abstract: | Summary Long-range physical maps of the small multigene family of the malt -amylase genes (-Amy-1) located on the long arms of wheat chromosomes 6A (the -Amy-A1 locus) and 6B (-Amy-B1) were generated by pulsed-field gel electrophoresis analysis. By using three methylation-sensitive rare-cutter restriction endonucleases, NotI, NruI and MluI, and an -Amy-1 cDNA probe and four gene-specific genomic probes from the -Amy-B1 locus, the size of the -Amy-B1 locus was estimated to be about 700 kb and of the -Amy-B1 locus to be about approximately 4300 kb. These two maps indicate clustering of GC-rich and C-methylation-sensitive restriction enzyme recognition sites. At least five regions reminiscent of CpG islands are apparent in -Amy-B1, and three in -Amy-A1. Correlation between recombination frequency and physical distance within the -Amy-B1 locus suggests that 1 cM approximates to 1 Mb in physical distance. |
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Keywords: | Wheat /content/kt5h2u32241w5476/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >-Amylase genes Restriction mapping Pulsed-field gel electrophoresis |
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