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Cloning and sequencing of the HU-2 gene of Escherichia coli |
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| Authors: | Yasunobu Kano Katsuaki Osato Morimasa Wada and Fumio Imamoto |
| Institution: | (1) Laboratory of Molecular Genetics, (Riken) Tsukuba Life Science Center, The Institute of Physical and Chemical Research, 3-1-1 Koyadai, Yatabe, 305 Tsukuba, Ibaraki, Japan;(2) Biotechnology Research Department, Research and Development Division, JGC corporation, 14-1 Bessho 1-chome, Minami-ku, 232 Yokohama, Japan |
| Abstract: | Summary The Escherichia coli HU-2 gene was cloned using a DNA fragment from the HU-1 gene as a probe. The amino acid sequence of the HU-2 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream of the translation initiation codon (AUG) and a possible rhoindependent terminater site downstream of the termination codon (UAA) of the gene. |
| Keywords: | Escherichia coli HU-2 protein Gene cloning Nucleotide sequence |
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