| 氮、锰、硫缺乏对蛋白核小球藻Chlorella pyrenoidosa光合产氢及其生长的影响 |
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| 引用本文： | 张磊,桑敏,李爱芬,张成武.氮、锰、硫缺乏对蛋白核小球藻Chlorella pyrenoidosa光合产氢及其生长的影响[J].生物工程学报,2010,26(4):489-494. |
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| 作者姓名： | 张磊 桑敏 李爱芬 张成武 |
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| 作者单位： | 暨南大学,水生生物研究中心,热带亚热带水生态工程教育部工程研究中心,广州,510632 |
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| 基金项目： | 珠海市科技攻关项目 (No. PC20081008) 资助。 |
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| 摘 要： | 本实验通过研究缺氮、缺锰和缺硫对蛋白核小球藻Chlorella pyrenoidosa产氢的影响,发现缺氮、缺锰及缺硫条件下该藻均能产氢,但在缺氮条件下产氢量最高,约为88.613μL H2/mgChla,分别是对照组、缺锰和缺硫实验组产氢量的4.61倍、1.92倍和3.63倍。通过对光合、呼吸及生长的比较研究,发现缺锰对该藻光合、呼吸及生长的影响要小于缺氮和缺硫;与正常培养条件相比,缺锰、缺硫抑制藻细胞的光合放氧和生长,对呼吸影响小,而缺氮不仅最大程度抑制光合放氧和生长,同时使呼吸作用增强,这为进一步优化该藻产氢条件及研究其产氢机制提供了线索。
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| 关 键 词： | 蛋白核小球藻，光合放氧，呼吸，产氢 |
| 收稿时间： | 2009/11/12 0:00:00 |
Effects of nitrogen, manganese, and sulfur deprivation on photo-hydrogen evolution and growth of Chlorella pyrenoidosa |
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| Lei Zhang,Min Sang,Aifen Li and Chengwu Zhang.Effects of nitrogen, manganese, and sulfur deprivation on photo-hydrogen evolution and growth of Chlorella pyrenoidosa[J].Chinese Journal of Biotechnology,2010,26(4):489-494. |
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| Authors: | Lei Zhang Min Sang Aifen Li and Chengwu Zhang |
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| Institution: | Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Miristry of Education, Institute of Hydrobiology, Jinan University, Guangzhou 510632, China;Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Miristry of Education, Institute of Hydrobiology, Jinan University, Guangzhou 510632, China;Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Miristry of Education, Institute of Hydrobiology, Jinan University, Guangzhou 510632, China;Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Miristry of Education, Institute of Hydrobiology, Jinan University, Guangzhou 510632, China |
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| Abstract: | We studied the hydrogen evolution(HE)of green alga Chlorella pyrenoidosa grown in normal(nutrients sufficient) media and nitrogen,manganese or sulfur deprived medium,The results showed that photo-hydrogen evolution could occur under all conditions hereinbefore,but the efficiency of HE was maximum under nitrogen deprivation,and the total hydrogen yield was 88.613 μL H_2/mg Chla,which was 4.61,1.92,3.63 times of control,manganese deprivation,sulfur deprivation groups,respectively.We also measured the growth,the photosynthesis and respiration of the alga.The data demonstrated that manganese deprivation had less influence than nitrogen and sulfur deprivation on the growth,the photosynthesis and respiration of C. pyrenoidosa.Compared with the normal(nutrients sufficient),manganese and sulfur deprivation inhibited the photosynthesis and growth ofthe alga while bringing small impact on respiration.Nitrogen deprivation,however,greatly restrained the photosynthesis and growth while enhancing the respiration.Those data provide clues for the further study on both the conditions optimization and mechanism of hydrogen evolution. |
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| Keywords: | Chlorella pyrenoidosa photosynthesis respiration hydrogen evolution |
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