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Properties of Photosynthetic Bacteria in Anisotropic Rigid Matrix and in Suspension |
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| Authors: | Planner A Hara M Stachowiak Z Miyake J |
| Institution: | (1) National Institute for Advanced Interdisciplinary Research, AIST/MITI, 1-1-4 Higashi, Tsukuba, Ibaraki, 305-8562, Japan;(2) Institute of Physics, Pozna , University of Technology, Piotrowo 3, 60-965 Pozna, Poland;(3) National Institute for Advanced Interdisciplinary Research, AIST/MITI, 1-1-4 Higashi, Tsukuba, Ibaraki, 305-8562, Japan;(4) Central Laboratory of Batteries and Cells, Forteczna 12/14, 61-362 Pozna, Poland |
| Abstract: | The photosynthetic bacteria (Rhodospirillum rubrum, Synechococcus and Anabaena variabilis) as well as their fragments embedded in isotropic and anisotropic polymer film were investigated. The orientation of photosynthetic pigments inside these organisms was compared, on the basis of the polarised absorption and fluorescence spectra, with the macroscopic orientation of investigated objects seen under microscope. The anisotropy of fluorescence was much higher than anisotropy of absorption. It showed strong influence of the photoselection by polarised radiation on the various bacterial chromophores exhibiting different orientations in the cells and various yields of fluorescence. The dimensions of cells were investigated on the basis of their photographs and by the scattering of the monochromatic radiation. |
| Keywords: | Anabaena variabilis cyanobacteria fluorescence microscope photographs orientation polarized spectra polyvinyl alcohol film purple bacteria radiation scattering Rhodospirillum rubrum Synechococcus |
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