马铃薯Y病毒N株HC-pro基因的序列分析与表达 |
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引用本文: | 王睿 吴云锋 郝兴安 付宏岐 杨栋 樊兵. 马铃薯Y病毒N株HC-pro基因的序列分析与表达[J]. Virologica Sinica, 2005, 20(3): 320-322 |
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作者姓名: | 王睿 吴云锋 郝兴安 付宏岐 杨栋 樊兵 |
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基金项目: | 国家自然科学基金(30270876) |
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摘 要: | The HC-pro gene was amplified by RT-PCR from total RNA of tobacco leaves infected with a N strain of Potato virus Y in Shaanxi, and cloned into the PMD 18-T vector. This HC-pro gene is consisted of 1371 nucleotides, encoding 457 amino acids. It shared the sequence homologyof 82.5%-96.4% nucleotide acid and 92.5%-98.0% in amino acids compared to 9 species of PVY N HC-pro abroad. The HC-pro gene was inserted into prokaryotic expressing vector pBV221, to obtain pBVHC recombinant plasmid in E. coli BL21. SDS-PAGE indicated that HC-pro proteins are successfully expressed in E. coli, Western blotting analysis demonstrated that the antibody against the expressed HC-pro can be used to identify the infected plants .
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关 键 词: | HC-pro基因 马铃薯Y病毒 序列分析 表达 |
Sequence Analysis of the HC-pro Gene of Potato Virus Y N Strain and its Expression in E .coli |
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Abstract: | |
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Keywords: | PVY-N HC-pro gene Prokaryotic expression |
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