A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors |
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Authors: | Huijing Zhang Haizhen Wu Huizhan Zhang |
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Institution: | (1) State Key Laboratory of Bioreactor Engineering (Mailbox 431), East China University of Science and Technology, Shanghai, 200237, China |
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Abstract: | A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance
marker and an l-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1,
pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system. |
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Keywords: | Plasmid vector pEC Compatible origin Copy number Stability Dual plasmids expression Gene regulation |
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