Development of resistant transgenic soybeans with inverted repeat-coat protein genes of soybean dwarf virus |
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Authors: | Makoto Tougou Noriyuki Furutani Noriko Yamagishi Yoshiaki Shizukawa Yoshihito Takahata Soh Hidaka |
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Institution: | (1) National Agricultural Research Center for Tohoku Region, Morioka Iwate, 020-0198, Japan;(2) Kyoto Prefectural Institute of Agricultural Biotechnology, Seika-cho, Soraku-Gun Kyoto, 619-0244, Japan;(3) Laboratory of Plant Breeding, Iwate University, Morioka Iwate, 020-8550, Japan |
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Abstract: | In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by β-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T0 plants with an introduced CP gene were obtained, and one generated T1 seeds. The presence of the transgene in T1 plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T2 plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T2 plants achieved resistance to SbDV by an RNA-silencing-mediated process. |
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Keywords: | Glycine max RNA silencing SbDV Transformation Resistance |
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