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生黑醋酸杆菌在高浓度山梨醇下的半连续发酵特性
引用本文:荆士华,汲涌,李春杰,姜铭妍,杨伟超,谌明,徐慧. 生黑醋酸杆菌在高浓度山梨醇下的半连续发酵特性[J]. 生物技术, 2009, 19(6): 77-78
作者姓名:荆士华  汲涌  李春杰  姜铭妍  杨伟超  谌明  徐慧
作者单位:1. 中国科学院沈阳应用生态研究所,辽宁沈阳,110016;东北制药总厂,辽宁沈阳,110026
2. 东北制药总厂,辽宁沈阳,110026
3. 中国科学院沈阳应用生态研究所,辽宁沈阳,110016
摘    要:目的:调节生黑醋酸杆菌生物和代谢特性,以提高发酵效率。方法:通过改变种液特性,采用半连续培养的方式,对生黑醋酸杆菌在高醇浓度下的生长特性进行了研究。结果:通过优化可以提高VC一步发酵底物山梨醇浓度达38%,32h左右发酵率达95%,山梨糖产量达360mg/ml,半连续培养连续5批之间产糖稳定,没有明显差别。结论:通过优化,有效地提高了山梨糖的产率。

关 键 词:发酵  山梨醇  L-山梨糖  种液

Studies on the Characteristic of Gluconbacter melanogenus with High Concentration of D-sordibol in Semi-continuous Fermentation
JING Shi-hua,JI Yong,LI Chun-jie,JIANG Min-yan,YANG Wei-chao,CHEN Ming,XU Hui. Studies on the Characteristic of Gluconbacter melanogenus with High Concentration of D-sordibol in Semi-continuous Fermentation[J]. Biotechnology, 2009, 19(6): 77-78
Authors:JING Shi-hua  JI Yong  LI Chun-jie  JIANG Min-yan  YANG Wei-chao  CHEN Ming  XU Hui
Abstract:Objective: To control the characteristic of Gluconbacter melanogenus in medium with high concentration of D-sordibol(38%). Method: by changing seed liquid and using semi-continuous fermentation.Result: The strain could be cultured well in medium containing D-sorbitol in 38 % . The conversion rate of D-sorbitol to L-sorbose in medium with 38 % D-sorbitol was 95 % over 30 hours. There was no notably different about the yields of L-sorbose in 5 generations fermentations continuously. Conclusion: The productivity of sorbose was improved notably.
Keywords:fermentation  sorbitol  L-sorbose  seed liquid
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