羊肚菌烂柄病发生对土壤真菌群落结构的影响

为了解羊肚菌(Morel)烂柄病的发生对土壤真菌群落结构的影响,采用Illumina MiSeq高通量测序技术,对健康羊肚菌根际土、烂柄病发病子实体根际土及相同环境下未栽培羊肚菌土壤的真菌群落结构进行研究。结果表明,测序样品共获得344 163条序列,归为7个真菌门。各样品真菌多样性分析结果表明,未栽培土壤真菌群落多样性较高,栽培羊肚菌根际土壤真菌多样性显著降低,烂柄病发生后土壤真菌多样性增加。群落结构分析表明,烂柄病根际优势真菌类群为拟青霉属(Paecilomyces)、木霉属(Trichoderma)、葡萄穗霉属(Stachybotrys)、枝顶孢属(Acremonium)、Paratritirachium、Zopfiella、被孢霉属(Mortierella)和柄孢壳属(Podospora)。烂柄病的发生改变了土壤真菌群落结构,促进了根际真菌的繁殖。为了解羊肚菌烂柄病的发生、传播机理及防治提供了参考。

Effects of Morel (Morchella sp.) Stipe Rot Disease Occurrence on Soil Fungal Community Structure

In order to understand the effect of the occurrence of morel (Morchella sp.) stipe rot disease on soil fungal community structure, Illumina MiSeq high-tflux sequencing technique was adopted to study fungal community structure of rhizosphere soil under the same conditions of healthy morel and stipe rot occurring morel as well as morel uncultivated soil. The results showed that a total of 344 163 valid sequences were acquired, which were assigned into 7 fungal phyla. The fungal diversity analysis results of various fungi showed that morel uncultivated soil had the highest diversity of soil fungal community, the fungal diversity of morel rhizosphere soil declined, and after the occurrence of stipe rot disease the soil fungal diversity increased. The community structure analysis suggested that the dominant fungal community of rhizosphere soil were Paecilomyces, Trichoderma, Stachybotrys, Acremonium, Paratritirachium, Zopfiella, Mortierella and Podospora. The occurrence of stipe rot disease changed the structure of the soil fungal community and promoted the propagation of rhizosphere fungus. The study provided a theoretical basis for understanding the occurrence, transmission mechanism and prevention of stipe rot disease on Morchella.