Ageing-Induced Alterations in Lipid/Phospholipid Profiles of Rat Brain and Liver Mitochondria: Implications for Mitochondrial Energy-Linked Functions |
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Authors: | Hiren R Modi Surendra S Katyare Minal A Patel |
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Institution: | (1) Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390 002, India |
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Abstract: | Effects of ageing on the lipid/phospholipid profile of brain and liver mitochondria from rats were examined. In the brain
mitochondria the contents of total phospholipid (TPL) and cholesterol (CHL) increased with simultaneous increase in the TPL/CHL
(mole:mole) ratio. The proportion and contents of lysophospholipid (Lyso), sphingomyelin (SPM), phosphatidylinositol (PI),
phosphatidylserine (PS) and diphosphatidylglycerol (DPG) components increased, with maximal increases seen for PS and PI;
phosphatidylcholine (PC) and phosphatidylethanolamine (PE) components registered decrease. In the liver mitochondria contents
of TPL and CHL increased. However, the TPL/CHL (mole:mole) ratio was not altered. Lyso, PI and PS increased. However, the
magnitude of increase was competitively lower; PE and DPG decreased. SPM and PC did not change as a consequence of ageing.
These changes altered the contents of individual phospholipids in the two membrane systems. Respiration with glutamate, pyruvate
+ malate, succinate and ascorbate + N,N,N’,N’-tetramethyl-p-phenylenediamine was significantly impaired in brain mitochondria from old animals. For liver mitochondria the respiratory
activity declined with glutamate and succinate. Correlation studies by regression analysis revealed that the lipid/phospholipid
classes regulate respiratory function differently in the mitochondria from the two tissues. The respiration-related parameters
in the brain mitochondria were dependent on multiple lipid/phospholipid components, and the process of regulation was complex
compared to the liver mitochondrial functions. |
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Keywords: | Age Brain mitochondrial lipid Liver mitochondrial lipid Brain mitochondrial phospholipid profile Liver mitochondrial phospholipid profile Mitochondrial respiration |
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