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Effects of neuropeptides on growth of cultivated rat molar pulp fibroblasts
Authors:U. Bongenhielm, A. H  gerstrand, E. Theodorsson,K. Fried
Affiliation:

a Department of Neuroscience, Karolinska Institutet, Doktorsringen 17, S-171 77, Stockholm, Sweden

b Department of Clinical Chemistry, Karolinska Hospital, S-171 77, Stockholm, Sweden

Abstract:The effect of the neuropeptides substance P (SP), neurokinin A (NKA), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) on DNA synthesis of dental pulp cells was investigated in cells grown from molar tooth bud explants from 4–6 days old rat pups. A concentration response-assay of the proliferative response of pulpal cells was performed with SP, NPY, NKA, CGRP and VIP (0.01 to 1 nM) in the presence of EGF (10 ng/ml), hydrocortisone (0.4 μg/ml) and 3% FCS, using [3H]thymidine incorporation. The results showed that SP, NKA and CGRP, but not NPY and VIP, increased the cell number in a concentration-dependent manner, with maxima at 10−10 – 10−9 M (SP, NKA) and 10−7 M (CGRP). No potentiating effect was noted when cells are simultaneously stimulated with SP and CGRP. The finding that SP, NKA and CGRP have growth regulatory properties on pulpal cells in vitro suggests that sensory neuropeptides may be involved during pulpal development or in wound healing after pulpal injury.
Keywords:Fibroblast   Rat   Dental pulp   Cell culture   Neuropeptide   Proliferation
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