The binding of cytochrome b5 to phosphatidylcholine vesicle |
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Authors: | M R Sullivan P W Holloway |
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Affiliation: | University of Virginia, School of Medicine, Department of Biochemistry Charlottesville, Virginia 22901 USA |
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Abstract: | Cytochrome b5 was isolated from rabbit liver by a detergent procedure and by a proteolytic procedure. Only cytochrome b5 isolated by the detergent procedure would bind to phosphatidylcholine vesicles and the cytochrome b5 was not removed by 1 M KCl. The Eo′ and visible absorption spectrum of the cytochrome b5 and its rate of reduction by NADH plus NADH-cytochrome b5 reductase did not change appreciably upon binding. These data indicate that cytochrome b5 is bound to phospholipid by a hydrophobic interaction which leaves the heme portion in the aqueous environment. |
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Keywords: | To whom correspondence should be addressed. |
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