Contraction of collagen by human fibroblasts and keratinocytes |
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Authors: | Jan E M Souren Maria Ponec Roeland van Wijk |
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Institution: | (1) Department of Molecular Cell Biology, State University, Padualaan 8, 3584 CH Utrecht;(2) Department of Dermatology, University Hospital Leiden, The Netherlands |
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Abstract: | Summary In the process of wound healing keratinocytes and fibroblasts play an important role, keratinocytes in the re-epithelization
process and fibroblasts in the process of wound contraction. We have studied the role of human keratinocytes and fibroblasts
in the rearrangement of collagen in a collagen lattice model system. Our results revealed that keratinocytes as well as fibroblasts
rearrange the collagen lattice; this occurs in a cell number and collagen concentration dependent manner. The optimal gel
contraction is obtained in the presence of keratinocytes on the top of and of fibroblasts in the collagen lattice, the situation
most closely approaching the in vivo situation. Between the two types of cells, differences in morphologic behavior were observed:
when incorporated into the gel the keratinocytes retained their spherical shape throughout the whole culture period, but fibroblasts
became elongated and formed extensions. Our data suggest that not only fibroblasts but also keratinocytes may be involved
in the process of wound contraction.
This work was supported by the Koningin Wilhelmina Fonds (Netherlands Cancer Foundation, grant 84-10). |
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Keywords: | collagen extracellular matrix wound healing fibroblasts keratinocytes |
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