Fish Faeces as the Primary Source of Chemical Cues Inducing Fish Avoidance Diapause in Daphnia Magna

In a laboratory batch culture experiment, females of Daphnia magna were exposed to five different experimental media containing either:(1) water from an aquarium with fish, (2) extract of fish faeces, (3) mixture of both media, (4) extract of homogenised conspecific Daphnia, or (5) control water without the addition of extra cues. The experiment was planned to test potential pathways of excretion of the chemical cues that induce resting-egg formation in D. magna and to find an effective way of collecting these chemical cues. The results indicate that fish faeces are the prevailing source of the chemical cues that induce resting-egg production in D. magna. The ease of collection and the possibility of storing it in a frozen state make it a convenient cue for inducing diapause response in Daphnia. The results of the experiment imply that in natural conditions Daphnia may face high concentration of the inductive signals once migrating to the bottom zone where fish faeces commonly accumulate.