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Plant regeneration from Bulgarian rose callus
Authors:Nahoko Ishioka  Shizufumi Tanimoto
Institution:(1) Genetic Engineering Laboratory, Faculty of Agriculture, Saga University, 840 Honjo, Saga, Japan
Abstract:Plant regeneration capacity of Bulgarian rose callus tissue was examined. Adventitious bud formation could be successfully attained, depending on the kinds of mineral salts used in the medium, auxin and cytokinin used. When callus tissues were cultured on the medium without ammonium nitrate and contained indoleacetic acid and benzyladenine, buds were formed in the callus. The number of buds were significantly increased by the simultaneous addition of calcium ionophore. When the cultures were transferred to the medium without cytokinin, roots were formed in the basal part of the buds.Abbreviations BA benzyladenine - IAA indoleacetic acid - K kinetin - NAA naphthaleneacetic acid
Keywords:adventitious bud  ammonium nitrate  calcium  Rosa damascena
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