Grouping of isolates in AG 2 ofRhizoctonia solani by total cellular fatty acid analysis |
| |
Authors: | Masaru Matsumoto Nobuaki Matsuyama |
| |
Institution: | (1) Institute of Tropical Agriculture, Kyushu University, 6-10-1, Hakozaki, 812-8581 Fukuoka, Japan;(2) Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, 812-8581 Fukuoka, Japan |
| |
Abstract: | Total-cellular fatty acid compositions of 34 isolates ofRhizoctonia solani belonging to intraspecific groups (ISGs) of anastomosis group (AG) 2, i.e., AG 2-1, AG 2-2 IIIB (mat rush), AG 2-2 IV (sugar
beet), AG 2-2 LP (turfgrass), and AG 2–3 (soybean), were compared. The major fatty acids identified were palmitic, stearic,
and oleic acids. Principal component analysis based on the percentage composition of total cellular fatty acids revealed consistently
low variability among isolates of a single ISG of AG 2. Average linkage cluster analysis showed that isolates obtained from
turfgrass representing a newly proposed group, AG 2-2 LP, were differentiated from other AG 2 ISGs. Isolates of another newly
proposed group AG 2–3, from diseased soybean were also closely related to AG 2-1 and AG 2-2 IIIB but distinguishable from
the AG 2-1 and AG 2-2 LP isolates by the average linkage cluster analysis. These results suggested that the percentage composition
of total-cellular fatty acids is a distinct characteristic for the five ISGs belonging to AG 2, and fatty acid analysis is
useful for the differentiation and characterization of these ISGs of AG 2 inR. solani. |
| |
Keywords: | fatty acid analysis PCR-RFLP analysis Rhizoctonia solani AG 2 |
本文献已被 ScienceDirect SpringerLink 等数据库收录! |
|