Plastid transformation in Arabidopsis thaliana |
| |
| Authors: | S R Sikdar G Serino S Chaudhuri Pal Maliga |
| |
| Institution: | (1) Waksman Institute, Rutgers, The State University of New Jersey 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA Fax: +1-732-4455735 e-mail: maliga@waksman.rutgers.edu, ML |
| |
| Abstract: | Plastid transformation is reported in Arabidopsis thaliana following biolistic delivery of transforming DNA into leaf cells. Transforming plasmid pGS31A carries a spectinomycin resistance
(aadA) gene flanked by plastid DNA sequences to target its insertion between trnV and the rps12/7 operon. Integration of aadA by two homologous recombination events via the flanking ptDNA sequences and selective amplification of the transplastomes
on spectinomycin medium yielded resistant cell lines and regenerated plants in which the plastid genome copies have been uniformly
altered. The efficiency of plastid transformation was low: 2 in 201 bombarded leaf samples. None of the 98 plants regenerated
from the two lines were fertile.
Received: 13 February 1998 / Revision received: 24 April 1998 / Accepted: 5 June 1998 |
| |
| Keywords: | aadA gene Arabidopsis thaliana Biolistic DNA delivery Plastid transformation Spectinomycin resistance |
| 本文献已被 SpringerLink 等数据库收录！ |
