Location and density labelling of acid invertase in aging discs of Daucus carota storage tissue |
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Authors: | John L Wray Richard E Brice |
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Institution: | Department of Biochemistry, University of St. Andrews, North Street, St. Andrews, Fife, Scotland |
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Abstract: | Cell walls prepared from aged discs by extraction in 0·1 M acetate buffer, pH 4·8, possess ionically bound acid invertase which can be removed from the wall by incubation in 1 M sodium chloride in 0·1 M acetate buffer, pH 4·8, and more firmly attached enzyme which is not removed. Cell walls prepared in 0·195 M phosphate-0·003 M citrate-buffer, pH 8·0, do not possess ionically bound enzyme. Ionically bound invertase is density labelled when discs are aged in 90% deuterium oxide suggesting that at least part of the increase in activity observed during aging is due to de novo protein synthesis. |
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Keywords: | Umbelliferae carrot root discs invertase cellular localization ionic binding density labelling |
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