Surface Display of GFP by <Emphasis Type="Italic">Pseudomonas Syringae</Emphasis> Truncated Ice Nucleation Protein in Attenuated <Emphasis Type="Italic">Vibrio Anguillarum</Emphasis> Strain |
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Authors: | Yuzhou Xu Qin Liu Lingyun Zhou Zhao Yang Yuanxing Zhang |
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Institution: | (1) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China |
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Abstract: | Microbial cell surface display of foreign proteins has been widely developed for many potential applications in live vaccine
construction, whole-cell biocatalysts, and bioadsorption. To investigate the feasibility of displaying heterologous proteins
on the surface of attenuated Vibrio anguillarum strain for potential multivalent live vaccine development, different display systems were built upon a truncated ice nucleation
protein (INP) from Pseudomonas syringae ICMP3023 whose N- and C-terminal domains were considered to be the putative membrane-anchoring motifs. Green fluorescent
protein (GFP), as a reporter, was fused with the display systems in different forms of N-GFP, NC-GFP, and N-GFP-C. Analysis
of the total expression level and surface localization of GFP demonstrated that the truncated P. syringae INP could be used to display foreign protein in V. anguillarum, while the system of N-GFP showed the higher levels of total expression and surface display based on unit cell density among
the three and might be available for further carrier vaccine development. |
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Keywords: | Bacterial cell surface display Ice nucleation protein Green fluorescent protein Vibrio anguillarum |
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