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梅花鹿源BVDV基因E0克隆与序列分析
引用本文:郜玉钢,李璠瑛,刘佳佳,杜锐,臧埔. 梅花鹿源BVDV基因E0克隆与序列分析[J]. 生物学杂志, 2009, 26(3): 4-6. DOI: 10.3969/j.issn.1008-9632.2009.03.004
作者姓名:郜玉钢  李璠瑛  刘佳佳  杜锐  臧埔
作者单位:吉林农业大学中药材学院,长春,130118
基金项目:国家自然科学基金,吉林农业大学博士启动基金 
摘    要:对梅花鹿源BVDV基因E0进行了克隆和序列分析。结果表明,梅花鹿源BVDV基因E0的大小为681bp,与报道9株BVDV(VEDEVAC、Bega、C24V、ILLC、NADL、OSLOSS、R1935、SD-1、Y546)和7株猪瘟病毒(ALD、Bres-cia、c、GPE、JL、LN9912、SM)及3株羊边界病毒(BD31、C413、BDVX818)相比,核苷酸序列的同源性依次为98.6%~84.8%、76.1%~74.7%、77.0%~76.7%。梅花鹿源BVDV为Ιb基因亚型。

关 键 词:梅花鹿  牛病毒性腹泻病毒  基因  克隆

Clone and sequence of BVDV E0 gene of sika deer
GAO Yu-gang,LI Fan-ying,LIU Jia-jia,DU Rui,ZANG Pu. Clone and sequence of BVDV E0 gene of sika deer[J]. Journal of Biology, 2009, 26(3): 4-6. DOI: 10.3969/j.issn.1008-9632.2009.03.004
Authors:GAO Yu-gang  LI Fan-ying  LIU Jia-jia  DU Rui  ZANG Pu
Affiliation:(Jinlin Agriculture University, Changchun 130118, China)
Abstract:Clone and sequence of BVDV E0 gene of sika deer were made. Results showed that the E0 gene of BVDV was 681bp. Compared with 9 strains BVDV (VEDEVAC, Bega, C24V, ILLC, NADL, OSLOSS, R1935, SD-1, Y546), 7 strains pestivirus (ALD, Brescia, c, GPE, JL, LN9912, SM), 3 strains border disease virus ( BD31, C413, BDVX818), the identities of nucleotide sequences were 98.6%-84. 8%, 76. 1% -74. 7% , 77% -76. 6%, respectively. The BVDV of sika deer was analyzed, it was classified to the type Ib.
Keywords:sika deer  bovine viral diarrhea virus  gene  clone
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