Transgenic fertile japonica rice plants expressing a modified cryIA(b) gene resistant to yellow stem borer |
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Authors: | C. Wu Y. Fan C. Zhang N. Oliva S. K. Datta |
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Affiliation: | (1) Plant Breeding Genetics and Biochemistry Division, International Rice Research Institute (IRRI), PO Box 933, Manila 1099, Philippines fax no: +63-2-8911292 E-mail: SDATTA@IRRI.CGNET.COM, XX;(2) Biotechnology Research Center, Chinese Academy of Agricultural Sciences, Beijing 100081, China, CN |
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Abstract: | The japonica rice variety Taipei 309 was cotransformed by particle bombardment of immature embryo-derived embryogenic calli with a modified δ-endotoxin gene cryIA(b) of Bacillus thuringiensis (Bt) under the control of the rice Actin1 promoter, and the hygromycin resistance gene, hph driven by the CaMV35S promoter. Selected transgenic rice plants showed enhanced insecticidal activity against yellow stem borer (Scirpophaga incertulas), with mortality rates reaching up to 100% in a bioassay with cut stems. Introduction and expression of the Actin1 promoter-Bt gene into rice provides japonica rice germplasm resistant to insect attack. Received: 21 March 1997 / Revision received: 23 June 1997 / Accepted: 5 July 1997 |
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Keywords: | Transgenic rice Particle bombardment cryIA(b) Yellow stem borer resistance |
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