Sequence comparison of mitochondrial tRNA genes and origin of light strand replication in Bos taurus and Nellore (Bos indicus) breeds |
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| Authors: | L Pegoraro Z Yang S Samaké F V Meirelles V Bordingnon L Moquin L C Smith |
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| Institution: | centre de recherche en reproduction animale (CRRA), Facultéde médecine vétérinaire, Universitiéde Montréal, Saint-Hyacinthe, Québec J2S 7C6 Canada;*EMBRAPA, Centro de Pesquisa de Pecuaria do Sudeste SaTo Carlos, SP, Brazil |
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| Abstract: | Although the complete bovine mitochondrial DNA molecule has been previously sequencedand sequence comparisons of the mitochondrial displacement loop have been performed, detailed sequence information is limited on coding regions of mitochondrial DNA within and among breeds of Bos taurus and Bos indicus. This study analysed polymorphism of the mitochondrial DNA transfer RNA genes for trypto-phan, alanine, asparagine, cysteine, tyrosine and the origin of light strand replication among Ayrshire, Canadian, Belgium Blue, Brown Swiss, Hereford, Jersey, Limousine, Piedmon-taise, Red Angus, Simmental (Bos taurus) and a Nellore (Bos indicus). Nucleotide sequence analysis of a 420-bp fragment of mitochondrial DNA comprising the five transfer RNA genes showed 100% homology among single individuals of the Bos taurus breeds. The Nellore breed showed guanine to adenine substitutions in the DHU arm of asparagine tRNA and in the origin of light-strand replication. This equates to a 0.5% sequence difference between the Nellore andBos taurus breeds and may reflect an independent evolutionary origin of the species. |
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| Keywords: | Bos taurus mitochondrial DNA Nellore polymorphism tRNA |
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