The pea <Emphasis Type="Italic">PsEND1</Emphasis> promoter drives the expression of GUS in transgenic wheat at the binucleate microspore stage and during pollen tube development |
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Authors: | Fernando Pistón Carmen García Gloria de la Viña José Pío Beltran Luis A Cañas Francisco Barro |
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Institution: | (1) Departamento de Agronomia y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, CSIC, Alameda del Obispo s/n, Cordoba, 14080, Spain;(2) NewBiotechnic, S.A, Bollullos de la Mitación, Sevilla, 41110, Spain;(3) Instituto de Biología Molecular y Celular de Plantas, CSIC-UPV, Campus de la Universidad Politécnica de Valencia, Av. de los Naranjos s/n, Valencia, 46022, Spain |
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Abstract: | Many plant genetic engineering applications require spatial expression of genes which in turn depends upon the availability
of specific promoters. In cereals, genetic modification of flowering and grain setting to influence yield and grain quality
is of significant interest. PsEND1 is a pea promoter that displays expression in the epidermis, connective tissue, endothecium and middle layers during different
stages of anther development. No homeologous sequence of this promoter or its coding sequence has been found in cereals. This
present work aimed at the characterization of the pea PsEND1 promoter driving the expression of the gusA gene in transgenic wheat. Nine transgenic lines were produced by particle bombardment and analyzed for the expression of
the gusA gene throughout development by histochemical GUS staining and by RT-PCR in vegetative and reproductive tissues and organs.
Expression of the gusA gene was first detected during pollen development, in microspores at binucleate stage. Activity of the gusA gene was also found in mature pollen, after anthesis. Following pollen grain germination, expression of the gusA gene was seen from an early stage of pollen tube formation until advanced stages, approaching the ovary. No further expression
of the gusA gene was detected after fertilization, nor during seed development. The results reported here show that the PsEND1 promoter is functional in wheat and its patterns of expression may be of interest for the application of genetic modification
in wheat breeding. |
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Keywords: | Cereal transformation Pollen embryogenesis PsEND1 promoter Wheat |
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