Secondary wall deposition in tracheary elements of cucumber grown in vitro |
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Authors: | S Kaliamoorthy KV Krishnamurthy |
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Institution: | (1) Department of Biotechnology, J.J. College of Arts and Science, Sivapuram, Namanasamudram P.O., Pudukkottai -, 622404 Tamil Nadu, India;(2) Department of Plant Science, Bharathidasan University, Tiruchirappalli -, 620024 Tamil Nadu, India |
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Abstract: | It is a matter of controversy whether secondary wall deposition is dependent on lignification during the development of tracheary
elements. To understand this, tracheary element differentiation was studied in the homogeneous calli obtained from the cotyledonary
explants of Cucumis sativus subsequent to treatment with plant growth regulators, such as naphthalene acetic acid (NAA) and
benzylamino purine (BAP), which are necessary for the induction of tracheary elements, along with metabolic blockers such
as 2-aminoindan-2-phosphonic acid (AIP), 2,3,5-triiodobenzoic acid (TIBA) and nifedipine. Calli treated with AIP, a potential
inhibitor of L-phenylalanine ammonia-lyase (PAL), have no PAL activity at any time during the culture period. There was a
complete inhibition of lignification although secondary wall deposition was unaltered. Similar results were obtained using
TIBA, an inhibitor of auxin transport, and nifedipine, a known calcium channel blocker. Thus the present study suggests that
secondary wall deposition in the course of tracheary element differentiation need not to be dependent on lignification.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | AIP Cucumis sativus lignification PAL TIBA xylogenesis |
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