Glycan microarray profiling of parasite infection sera identifies the LDNF glycan as a potential antigen for serodiagnosis of trichinellosis |
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Authors: | Aranzamendi Carmen Tefsen Boris Jansen Montse Chiumiento Lorena Bruschi Fabrizio Kortbeek Titia Smith David F Cummings Richard D Pinelli Elena Van Die Irma |
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Affiliation: | aCentre for Infectious Disease Control Netherlands, National Institute of Public Health and the Environment, Bilthoven, The Netherlands;bDepartment of Molecular Cell Biology & Immunology, VU University Center, Amsterdam, The Netherlands;cDepartment of Experimental Pathology BMIE, Medical School, Università di Pisa, Italy;dDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA |
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Abstract: | Diagnostic methods for parasite infections still highly depend on the identification of the parasites by direct methods such as microscopic examination of blood, stool and tissue biopsies. Serodiagnosis is often carried out to complement the direct methods; however, few synthetic antigens with sufficient sensitivity and specificity are available. Here we evaluated a glycan microarray approach to select for synthetic glycan antigens that could be used for serodiagnosis of parasitic infections. Using a glycan array containing over 250 different glycan antigens, we identified GalNAcβ1–4(Fucα1–3)GlcNAc-R (LDNF) as a glycan antigen that is recognized by antibodies from Trichinella-infected individuals. We synthesized a neoglycoconjugate, consisting of five LDNF molecules covalently coupled to bovine serum albumin (BSA), and used this neoglycoconjugate as an antigen to develop a highly sensitive total-Ig ELISA for serological screening of trichinellosis. The results indicate that glycan microarrays constitute a promising technology for fast and specific identification of parasite glycan antigens to improve serodiagnosis of different parasitic infections, either using an ELISA format, or parasite-specific glycan arrays. |
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Keywords: | Serology Glycosylation Helminth Glycan microarray Trichinella spiralis |
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