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Low-pass sequencing for microbial comparative genomics |
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| Authors: | Ah Young Goo Jared Roach Gustavo Glusman Nitin S Baliga Kerry Deutsch Min Pan Sean Kennedy Shiladitya DasSarma Wailap Victor Ng Leroy Hood |
| Institution: | (1) Institute for Systems Biology, 98103 Seattle, Washington, USA;(2) Center of Marine Biotechnology, University of Maryland Biotechnology Institute, 21202 Baltimore, Maryland, USA;(3) Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Taipei, Taiwan |
| Abstract: | BackgroundWe studied four extremely halophilic archaea by low-pass shotgun sequencing: (1) the metabolically versatile Haloarcula marismortui; (2) the non-pigmented Natrialba asiatica; (3) the psychrophile Halorubrum lacusprofundi and (4) the Dead Sea isolate Halobaculum gomorrense. Approximately one thousand single pass genomic sequences per genome were obtained. The data were analyzed by comparative genomic analyses using the completed Halobacterium sp. NRC-1 genome as a reference. Low-pass shotgun sequencing is a simple, inexpensive, and rapid approach that can readily be performed on any cultured microbe. |
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