The effect of cdk-5 overexpression on tau phosphorylation and spatial memory of rat |
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Authors: | Xiaomei?Liao Yingchun?Zhang Yipeng?Wang Email author" target="_blank">Jianzhi?WangEmail author |
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Institution: | Department of Pathophysiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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Abstract: | In Alzheimer’s disease (AD), hyperphosphorylation of tau may be the underlying mechanism for the cytoskeletal abnormalities
and neuronal death. It was reported that cyclin-dependent kinase5 (cdk-5) could phosphorylate tau at most AD-related epitopesin vitro. In this study, we investigated the effect of cdk-5 overexpression on tau phosphorylation and spatial memory in rat. We demonstrated
that 24 h after transfection into rat hippocampus, cdk-5 was overexpressed and induced a reduced staining with antibody tau-1
and an enhanced staining with antibodies 12e8 and PHF-1, suggesting hyperphosphorylation of tau at Ser199/202, Ser262/356
and Ser396/404 sites. Additionally, the cdk-5 transfected rats showed long latency to find the hidden platform in Morris water
maze compared to the control rat. 48 h after transfection, the level of cdk-5 was decreased significantly, and the latency
of rats to find the hidden platform was prolonged. It implies thatin vivo overexpression of cdk-5 leads to impairment of spatial memory in rat and tau hyperphosphorylation may be the underlying mechanism. |
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Keywords: | cyclin-dependent kinase5 (cdk-5) tau spatial memory Alzheimer's disease |
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