慢性间歇低氧对幼鼠脑区p38MAPK的影响

目的：研究慢性间歇低氧对幼鼠部分脑区p38MAPK的影响。方法：SPF级健康雄性SD幼鼠（3-4周龄）50只,随机分为5组（n=10）：间歇低氧2周组（2IH组）、间歇低氧4周组（4IH组）、间歇低氧4周后恢复组（4F组）、对照2周组（2C组）和对照4周组（4C组）。建立慢性间歇低氧幼鼠模型,以RT-PCR法和Westemblot法分别测幼鼠海马及前额叶皮层p38MAPKmRNA和磷酸化p38MAPK（p-p38）蛋白的表达。结果：21H、4IH和4F组幼鼠海马、前额叶皮层的p38MAPK mRNA及p-p38蛋白均明显高于相应对照组（P均〈0．05）。结论：慢性间歇低氧可激活幼鼠部分脑区D38MAPK。

The effect of chronic intermittent hypoxia on p38MAPK in cerebral tissues of weanling rats

Aim： To study the effect of chronic intermittent hypoxia an p38MAPK in partial cerebral tissues of weanling rats. Methods： Randomly, fifty male SD rats（3-week-old- 4-week-old） were divided into five groups： 2-week-CIH group（2IH）, 4-week-CIH group（4IH）, 4-week-recovery group（4F）, 2-week-control group（2C） and 4-week-control group（4C）. Intermittent hypoxia model was induced by an intermittent hypoxia cabin. The expression of p38MAPK mRNA and the phosphorylation levels of p38MAPK（p-p38 protein） in the hippocampus and prefrontal cortex were measured by RT-PCR or Western blot respectively. Results： No matter in the hippocampus, or in the prefrontal cortex, the expression of p38MAPK mRNA and p-p38 protein in 2IH, 4IH and 4F groups were respectively higher than 2C and 4C groups（ P 〈 0.05, respectively）. Conclusion： Chronic intermittent hypoxia can activate the p38MAPK in partial cerebral tissues of weanling rats.