运动训练对大鼠主动脉应力和NF-κB及c-fos表达的影响

目的：探讨不同强度运动训练对大鼠主动脉应力和NF-κB及c-fos表达的影响。方法：采用跑台训练方式,建立大鼠有氧运动和疲劳运动模型,运用免疫组织化学SABC法研究不同强度运动训练对大鼠主动脉VEC和VSMC中NF-κB及c-fos表达的影响。结果：胸主动脉血压的变化与对照组比较,有氧训练和疲劳训练均显著性升高（P〈0．05）,但有氧训练与疲劳训练之间差异不显著。与对照组比较,有氧训练大鼠VEC和VSMC中NF-κB和c-fos均显著性下调表达,胸主动脉张开角显著性升高（P〈0．05）,疲劳训练大鼠VEC和VSMC中NF-κB和c-fos均显著性上调表达（P〈0．05）。与有氧运动组比较,疲劳运动大鼠VEC和VSMC中NF-κB和c-fos表达与胸主动脉张开角升高均具有显著性差异（P〈0．05）。表明不同强度运动大鼠主动脉VEC中NF-κB和c-fos表达变化趋势不同,疲劳训练组表达的增加趋势更显著。结论：运动可引起大鼠主动脉VEC和VSMC NF-κB及c-fos表达的变化,且与运动强度关系密切。有氧训练引起的慢性剪切应力变化可使主动脉VEC和VSMC NF-κB加及c-fos显著性下调表达,与VEC和VSMC维持血管功能的稳态有关;疲劳训练可导致壁面摩擦剪切力、周向应力增加,过度的剪切力作用可引起主动脉VEC和VSMC NF-κB及c-fos显著性上调表达,血管张开角显著增加,血管发生非均匀生长,引起血管结构与功能的重塑。

Effects of exercises training on the rats aorta stress and expression of NF-kappaB and c-Fos

Aim： To investigate the effect of different intensity exercise training on the rat aorta stress and expression of NF-κB and c-fos. Methods： We used treadmill exercise to establish the rat models of aerobic exercise and fatigue exercise, applied immunohistochemistry of SABC to measure the effect of different intensity exercise training on expression of NF-κB and c-fos in the VEC and VSMC of rat aorta. Results： The change of aorta blood pressure, compared with the control group, which had significant increase in both aerobic exercise and fatigue exercise （ P 〈 0.05）, but there had no remarkable difference between in aerobic exercise and fatigue exercise. Compared with the control group, the expression of NF-κB and c-fos of VEC and VSMC in aerobic exercise rats remarkably reduced, the opening angle increased remarkably（ P 〈 0.05）, and the expression of NF-κB and c-fos of VEC and VSMC in fatigue exercise rats distinguished upregulated（ P 〈 0.05）. Compared with the aerobic training group, there was distinguished difference in both the expression of NF-κB and c-fos of VEC and VSMC and the opening angle of pectoralis aorta in fatigue training rats（ P 〈 0.05）. This indicated that different intensity training had different effect on the expression of NF-κB and c-fos of VEC and VSMC of rat aorta, and the increase trend of expression of fatigue training group was more remarkably. Conclusion： Expression change of NF-κB and c-fos of VEC and VSMC of rat aorta was caused by exercise training, and which had a closed relation with the exercise intensity. Chronic shear stress of aerobic training made the expression of NF-κB and c-fos of VEC and VSMC of aorta distinguished downregulated, which had relationship with homeostasis that VEC and VSMC maintained the blood vessel function. Fatigue training caused the increase of wall frictional shear stress and circumferential stresses, excessive shear stress caused the expression of NF-κB and c- fos of VEC and VSMC of aorta significant upregulated,