Activation of chemical defenses in the tropical green algae Halimeda spp.

Halimeda spp. are among the most common seaweeds on tropical reefs where herbivory is intense. These calcified seaweeds produce diterpenoid feeding deterrents; the major metabolites are halimedatetraacetate and halimedatrial. We found that most species of Halimeda on Guam immediately convert the less-deterrent secondary metabolite halimedatetraacetate to the more potent feeding deterrent halimedatrial when plants are injured by grinding or crushing. This conversion would therefore occur when fishes bite or chew Halimeda plants. We term this process of rapid conversion “activation”. Extracts from injured plants contained higher amounts of halimedatrial and were more deterrent toward herbivorous fishes than extracts from control plants. Herbivore-activated defenses are common in many families of terrestrial plants: however, this is the first example of an activated defense in a marine plant.