Use of high culture pH for the decreased proteolysis of human parathyroid hormone secreted by Saccharomyces cerevisiae |
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Authors: | Gu Yong Song Bong Hyun Chung |
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Affiliation: | (1) Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon, 305-600, Korea |
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Abstract: | Human parathyroid hormone (hPTH) was expressed and secreted in Saccharomyces cerevisiae. In batch fermentations performed at pH = 5.6, 6.5, 7.2 and 7.5, optimal production of hPTH (12.1 mg/l) was obtained at pH 7.2 after 24 h of culture. At pH 5.6, most of secreted hPTH was degraded. Proteolysis of hPTH was significantly decreased by increasing the culture pH. |
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Keywords: | human parathyroid hormone culture pH proteolysis Saccharomyces cerevisiae |
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