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Several regions of the repeat domain of the Staphylococcus caprae autolysin,AtlC, are involved in fibronectin binding
Authors:Allignet Jeanine  England Patrick  Old Iain  El Solh Névine
Institution:Department of Bacteriology, School of Medicine, The University of Tokushima, Tokushima 770-8503, Kuramoto-cho, Japan.
Abstract:The chromosome of Bacteroides fragilis strain YCH46 was shown to be a single circular DNA molecule of about 5.3 Mb having 16 NotI, seven AscI, and six I-CeuI sites. A physical map of the chromosome was constructed by four independent experimental approaches: linking clone analysis, cross-Southern hybridization, partial restriction digestion, and two-dimensional pulsed-field gel electrophoresis. Six rRNA operons and 10 known genes were localized on the physical map.
Keywords:Physical map  Pulsed-field gel electrophoresis              Bacteroides fragilis
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