PCR method for detecting recombinant baculoviruses with the insertion of a large gene |
| |
| Authors: | Lai Su-Yuan Ho Jin-Yi Wang Min-Ying |
| |
| Affiliation: | (1) Institute of Biochemical Science, National Taiwan University, Taipei, Taiwan;(2) Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung, Taiwan |
| |
| Abstract: | A PCR strategy was developed using primers specific to an infectious bursal disease virus (IBDV) gene as well as primers flanking the polyhedrin region of baculovirus to verify the presence of IBDV gene in the recombinant baculovirus and confirm the absence of wild-type baculovirus contamination. This method can be applied to detect the presence of large genes in the recombinant baculovirus with greater sensitivity and avoid the need of modifying the typical PCR procedure provided by the manufacturer. © Rapid Science Ltd. 1998 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
