Coexistence of fast-type and slow-type C-proteins in neonatal chicken breast muscle |
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| Authors: | T Obinata S Kitani T Masaki D A Fischman |
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| Institution: | 1. Department of Biology, Chiba University, Yayoi-cho, Chiba-shi, Chiba 260, Japan;2. Department of Cell Biology and Anatomy, Cornell University Medical College, 1300 York Avenue, New York 10021 USA |
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| Abstract: | Two different C-protein variants which selectively react with either monoclonal anti-fast C-protein antibody (MF-1) or monoclonal anti-slow C-protein antibody (ALD-66) were separated from neonatal chicken pectoralis muscle by hydroxylapatite column chromatography. Myofibrils isolated from the neonatal chicken muscle reacted with both monoclonal antibodies as examined by an indirect immunofluorescence method. These observations strongly indicate that both fast-type and slow-type C-proteins are expressed in the neonatal chicken skeletal muscle. Both of them are intermingled and assembled in the same myofibrils. |
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| Keywords: | To whom correspondence and reprint requests should be addressed |
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