Glycollate oxidase inhibition and its effect on photosynthesis and pigment formation in Zea mays |
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| Authors: | C.L.D. Jenkins L.J. Rogers M.W. Kerr |
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| Affiliation: | 1. Department of Biochemistry, University College of Wales, Aberystwyth, SY23 3DD, Wales, U.K.;2. Shell Research Limited, Sittingbourne Research Centre, Sittingbourne, Kent, ME9 8AG, U.K. |
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| Abstract: | We have investigated the effect of 2-hydroxy-3-butynoic acid (HBA) and its methyl ester (MeHBA) on photosynthesis and pigment formation in Zea mays, a C4 photosynthesis-type plant. In the presence of the specific inhibitor of glycollate oxidase, assimilation of CO2 was decreased significantly. Labelling patterns showed accumulation of glycollate, though not so marked as in C3 photosynthesis-type plants, and marked decreases in incorporation into glycine, serine and particularly glycerate. This inhibition was specific for the S(+) enantiomers of HBA and MeHBA. In greening maize R,S-MeHBA inhibited formation of chloroplast pigments and this effect could be shown to be due to the S(+) enantiomer; of a range of metabolises tested only supplementations with serine or pyruvate were partly effective in restoring greening. |
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| Keywords: | Gramineae maize glycollate oxidase photorespiration photosynthesis 2-hydroxy-3-butynoic acid pigment formation greening. |
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