Arabinogalactans in a purified allergen preparation from pollen of timothy (Phleum pratense) |
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| Authors: | Svein Haavik Berit Smestad Paulsen Jens K Wold Øivind Grimme |
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| Institution: | 1. >Department of Pharmacognosy, Institute of Pharmacy, University of Oslo, P.O. Box 1068, Blindern, Oslo 3, Norway;2. Bioanalytical Department, Nyegaard & Co. A/S, Oslo 4, Norway |
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| Abstract: | The purified allergen preparation representing a certain fraction of an aqueous timothy pollen extractcontained ca. 20% carbohydrate, mainly as arabinose (7%) and galactose (13%). The protein content was 63%. Fractionation on DEAE-Sephadex and Sephadex G-100 gave one neutral and two acidic fractions, all containing protein, arabinose and galactose. The structure of the carbohydrate moiety was investigated by methylation analysis, periodate oxidation and enzyme incubation. The acidic fraction contained (1→6)-linked galactose residues, some being substituted on O-3 with arabinose. The neutral fraction consisted of a more extensively branched arabinogalactan with longer side chains of (1→3)- and (1→5)-linked arabinose. The arabinose was present mainly as α-l-arabinofuranosyl residues. Alkaline degradation and subsequent fractionation indicated the presence of a covalent linkage between hydroxyproline and arabinose. Periodate oxidation or incubation with α-l-arabinofuranosidase did not affect the allergenic activity of the extract. |
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| Keywords: | Poaceae timothy grass pollen allergen preparation arabinogalactan glycoprotein hydroxyproline |
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