Entrapment of lipase in silica glass by the sol-gel method and its esterification activity in organic media |
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Authors: | Koei Kawakami Shigehiro Yoshida |
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Affiliation: | (1) Department of Chemical Engineering, Kyushu University, Hakozaki Higashi-ku, 812 Fukuoka, Japan |
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Abstract: | Summary Silica glass-entrapped lipase was prepared by the sol-gel method using tetramethoxysilane, and its esterification activity in n-hexane was examined for isoamylbutyrate formation. The hydrogel preparation containing a large amount of water exhibited enough activity. Although the activity of xerogel-entrapped lipase drastically decreased probably due to shrinkage of the gel matrix, the lyophilized gel retained much higher activity than the air-dried gel. |
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