Serological study of yeast killer toxins by monoclonal antibodies |
| |
| Authors: | Luciano Polonelli Stefania Manzara Stefania Conti Giuseppe Dettori Giulia Morace Carlo Chezzi |
| |
| Institution: | (1) Istituto di Microbiologia, Universitá di Parma, Via Gramsci 14, 43 100 Parma, Italy;(2) Institute di Microbiologia, Universtá Cattolica del Sacro Cuore, Largo F Vito, 1 - 00168 Rome, Italy |
| |
| Abstract: | Yeast killer toxins coded by determined and undetermined killer plasmids or presumptive nuclear gene(s) in various genera (Saccharomyces, Kluyveromyces, Pichia and Candida) have been serologically investigated by a monoclonal antibody (KT4), produced against the yeast killer toxin of Pichia (Hansenula) anomala UCSC 25F. Double immunodiffusion with the killer toxins as antigens and indirect immunofluorescence on whole cells of the corresponding killer yeast have been used. In both the serological procedures, monoclonal antibody KT4 proved to be reacting only with the killer toxins and the whole cells of yeasts belonging to the genus Pichia. |
| |
| Keywords: | yeast killer toxins monoclonal antibodies immunodiffusion indirect immunofluorescence |
| 本文献已被 SpringerLink 等数据库收录！ |
|
