Ontogeny of alpha-crystallin subunits in the lens of human and rat embryos |
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Authors: | Masami Oguni Tomoichi Setogawa Ryuju Hashimoto Osamu Tanaka Haruo Shinohara Kanefusa Kato |
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Affiliation: | (1) Department of Anatomy, Shimane Medical University, 693 Izumo, Japan;(2) Department of Ophthalmology, Shimane Medical University, 693 Izumo, Japan;(3) Department of Anatomy, School of Medicine, Mie University, 514 Tsu, Japan;(4) Department of Biochemistry, Institute for Developmental Research, 480-03 Aichi Prefectural Colony, Kasugai, Japan |
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Abstract: | The distribution of A- and B-crystallin in the developing lens of human (Carnegie stages 13 to 23) and rat embryos (embryonic days E11 to 18) was examined immunohistochemically. In a human embryo at stage 13, the lens placode was already immunoreactive to B-crystallin, but not to A-crystallin. At stage 15, the lens vesicle was intensely immunoreactive both to A- and B-crystallin. From stages 16 to 23, the lens epithelial cells and fiber cells were immunoreactive to A- and B-crystallin. In rat embryos, A-crystallin appeared in the lens pit at E12, and B-crystallin appeared in the elongating lens fiber cells at E14. From E15 to E18, the lens epithelial cells and fiber cells were immunoreactive to A-crystallin. The lens fiber cells were also immunoreactive to B-crystallin, but the epithelial cells were not. These findings suggest that B-crystallin appears earlier than A-crystallin in the human lens, but at a later period than A-crystallin in the rat lens. B-Crystallin was not detected in the epithelial cells of the rat lens, but was perisistently present in the epithelial cells of the human lens. |
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Keywords: | Eye Lens Development, ontogenetic /content/n38t5146142449u2/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >A-crystallin /content/n38t5146142449u2/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >B-crystallin Immunohistochemistry Human Rat (Wistar) |
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