Effort to reconstruct past population history in the fern <Emphasis Type="Italic">Blechnum spicant</Emphasis> |
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Authors: | Helena Korpelainen Maria Pietiläinen |
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Institution: | (1) Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014 Helsinki, Finland |
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Abstract: | Our aim was to evaluate the potential of existing herbarium collections in reconstructing the past population history of the
rediscovered clone of the regionally extinct Blechnum spicant by comparing it with herbarium material from previous sites of occurrences in Finland and elsewhere in northern Europe, as
well as to reveal the genetic and geographic relationship among the samples. We detected a total of nine polymorphic sites
within the three sequenced regions, two SCAR markers developed now for B. spicant and the chloroplast trnL-trnF spacer, totalling 763 bp. Despite low variability, the phylogeographic analysis revealed the presence of some geographic
pattern among the samples, of which all except one represented herbarium samples of up to 100 years of age. The rediscovered
clone of B. spicant proved to represent the prevalent genotype occurring in northern Europe. The studied sequences were neutral in terms of evolution.
It is apparent that existing herbarium collections are useful resources for a range of evolutionary and population studies. |
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Keywords: | Blechnum spicant Blechnaceae Herbarium specimens Phylogeographic analysis Population history Sequence diversity |
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